Biology Protocols

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  • Product Reviews
    • WinDrawChem - Chemical Drawing Package
    • BVTech Plasmid - DNA plasmid drawing software
    • Geospiza FinchTV - DNA sequence trace viewer
    • The Ultimate Lab Pen? (A review of the DANE-ELEC Zpen)
  • DNA analysis
    • Restriction Fragment Mapping
    • Agarose gel electrophoresis
  • Protein Analysis
    • Limited Proteolysis of proteins
    • Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)
  • Liquid chromatography purification of proteins
    • Affinity chromatography using immobilised glutathione
  • Molecular Biology
    • Ligation of DNA molecules
• Protocols
  • Bacterial Growth Media Recipes
    • Autoinduction Media Protocol
    • Autoinduction Media Components
    • Minimal Medium
    • Growth Media and Induction protocol for production of biotinylated proteins (Avidity.com)
    • SOC Media (1 L)
    • Terrific Broth
    • 2X YT Media Recipe
    • LB (Luria-Bertani) Media - Plates
    • LB (Luria-Bertani) Media - Broth
  • Gel Electrophoresis and related
    • 10 X Blue Native Loading dye
    • SDS-PAGE buffers and solutions
    • Using the correct precentage acrylamide for SDS-PAGE
  • Molecular Biology Protocols
    • 10X Triplex Annealing Buffer
    • Phenol/Chloroform Extraction of DNA
    • Preparation of competent cells Calcium Chloride method
    • Medium Scale Rapid Boiling Method
    • A Practical set of recommendations for ligation
    • Extraction of DNA from agarose gels using filter tips
    • Extraction of DNA from agarose gels using rapid freezing
    • Ethanol Precipitation of Nucleic Acids
    • Purification of DNA from Agarose by Electrophoresis
    • Large Scale Prep for Very Large Plasmids and Cosmids
    • PEG Purification of Plasmids
    • Rapid Large Scale Isolation of Plasmid DNA (based on Promega protocol)
    • Miniprep Procedure for Plasmids (based on Birnboim and Doyle method)
    • Large Scale Plasmid Preparation
    • Brij-Doc Lysis Plasmid Extraction
• Calculators
  • Sequence Manipulation Tools
    • Sequence Manipulation Suite 2
  • Rotor RCF to RPM Calculators
    • RCF Java Applet
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    • Agarose Gel Calculator
    • SDS-PAGE calculator (30% acrylamide stock)
    • SDS-PAGE calculator (40% acrylamide stock)
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