Triplex DNA can be used as substrates for the analysis of a variety of DNA motors, various triplex forming sequences have been identified and once formed can be exchanged into a desired buffer for analysis/assay.
Recipe:
| Consituent | For 50 ml |
| 25 mM MES, pH 5.5 | 1.25 ml (1M Stock) |
| 10 mM MgCl2 | 0.5 ml (1M Stock) |
Protocol:
To anneal, mix equimolar concentrations of linear (dsDNA duplex) and TFO in a 1X concentration of the above buffer. Heat to 57˚C for 15 minutes and slowly cool to room temperature (preferably overnight).
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