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SDS-PAGE calculator (40% acrylamide stock)

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Tris-glycine SDS-Polyacrylamide Gel (SDS-PAGE Calculator for 40% acrylamide stocks)
Stacking Separating
Gel percentage (%) Gel percentage (%)
40% Polyacrylamide (mL) 40% Polyacrylamide (mL)
1M Tris(pH6.8)(mL) 1.5M Tris(pH8.8)(mL)
10% Ammonium persulfate (mL) 10% Ammonium persulfate (mL)
10% SDS (mL) 10% SDS (mL)
TEMED (mL) TEMED (mL)
H2O (mL) H2O (mL)
Total volume (mL) Total volume (mL)
(Author = http://www.changbioscience.com )

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